DHTKD1 and neoplasm: Analysis of viral E1A DNA produced by Ad-TD-nsIL-12 in tumor or normal tissues using this model further supported the tumor specificity of the nsIL-12-armed adenovirus at a dose of 2.5 × 109 PFU/injection, with E1A being detected at much lower levels (close to the limit of assay sensitivity) in normal lung or liver tissue compared to tumor tissue (Fig. 6e).