BRAF gene mutational analysis in the 41 metastatic melanoma tissues, identified the V600E BRAF mutation in 19 metastases (19/41, 46.3%), with 22 samples (22/41, 53.7%) characterized as wild-type BRAF. Eleven (11/41, 26.8%) wild-type BRAF patients were not suitable for immune check-point therapy, three of which (3/41, 7.3%) were in disease progression after Ipilimumab immunotherapy and 8 of which (19.5%) were ineligible due to hepatitis C (4 patients; 9.7%), human immunodeficiency virus-HIV (one patient; 2.4%) and active inflammatory bowel disease (three patients; 7.3%). This evidence concerns the gene BRAF and melanoma.