Major findings in this study demonstrated that the trans-generational impact of F0 maternal HF diet on the F2 generation such that the F2 offspring had an increased body weight, glucose intolerance, β-cell function and proliferation impairment, along with a decreased expression of Pdx1 and p-NeuroD1, despite being fed with the SC diet after weaning. This evidence concerns the gene NEUROD1 and hydrops fetalis.