Notably the concordant epigenetic changes of H2A.Zac nucleosome occupancy, chromatin accessibility, DNA methylation and gene expression, were observed at promoters and enhancers of cancer-related genes that are overexpressed in LNCaP cells (Fig. 5a and Supplementary Fig. 4a) and in clinical prostate cancer, GREB1, TRPM8, KLK2, and KLK327. This evidence concerns the gene TRPM8 and prostate cancer.