ASNS and cancer: We used asparagine synthetase (ASNS) to assess the levels of asparagine synthesis in the cancer versus control tissues (Fig. 4g) and found that asparagine synthesis is up-regulated in 10 types of cancer (except for PRAD), among which BLCA and LUAD have increased contributions to asparagine from glutamine; BRCA, KIRC, KIRP, LIHC, and THCA have decreased contributions from glutamine; and COAD, HNSC, and KICH have slightly changed contributions from glutamine (Fig. 5g).