We found that the CD8+ T cells displayed the most profound impairment in production of IL-2 (data not shown); however, the STAT3-blocked HCC vaccine improved the production of TNF-α and IFN-γ by CD8+T cells (Fig. 6b), as well as the percentage of perforin-expressing CD8+T cells. This evidence concerns the gene CD8A and hepatocellular carcinoma.