Third, in light of the observation that miR-193a-3p inhibited the invasion ability of the cancer cells, we analyzed the effect of miR-193a-3p overexpression on expressions of epithelial-mesenchymal-transition-related (EMT-related) genes such as ZEB1, ZEB2, SNAI1, and SNAI2. We found that the expression of ZEB1, SNAI1, and SNAI2 was decreased in RKO cells transfected with the pre-miR-193a-3p compared to those transfected with a negative control (Fig. 3d), while the expression of ZEB2 was not detected in RKO cells transfected with the negative control or those transfected with pre-miR-193a-3p. The gene discussed is SNAI2; the disease is cancer.