For validation of predicted targets for microRNAs deregulated in metastatic canine mammary cancer were selected 14 genes, which expression differ in the metastatic and non-metastatic group (CDC6, CCNE1, MYBL2, PDCD10, ERBB2IP, SON, STK4, CDC27, PRC1, CDC37, TTK, SKIL, BUB3 and SPIN1). The gene discussed is CCNE1; the disease is breast cancer.