CD4 and myasthenia gravis: Although the method is more laborious and time consuming than the whole PBMC or CD8+-depleted PBMC culture methods, it allows for a higher CD4+ T cell density to be screened for peptide recognition, which has merits when the frequency of responsive CD4+ T cells is low as in MG patients (34, 35) Moreover, the antigen-presenting cell (APC) function is dependent on moDCs in DC:TC co-cultures and since DC frequencies are very low (1%) in blood the APC function relies primarily on monocytes and B cells, and not DCs, in the PBMC cultures (38).