Kallergi, G et al. investigated the expression of the EMT markers Twist 1 and vimentin in CTCs from 25 patients with metastatic and 25 patients with early breast cancer and identified Twist 1(+) and vimentin(+) CTCs in 73% and 77% of patients with early breast cancer, respectively, whereas CTCs from all patients with metastatic breast cancer were positive for these markers [125]. This evidence concerns the gene VIM and breast carcinoma.