Furthermore, when the depleted B7-H4 HCC cells were cocultured with allogeneic PBMCs, the blockade of B7-H4 increased the expression of CD8+ T cell effector cytokines Granzyme A (p=0.023), Granzyme B (p=0.006), perforin (p=0.030) and IFN-γ (p=0.011) (n=5, Figure 4D). This evidence concerns the gene PRF1 and hepatocellular carcinoma.