As shown in Figure 2C, consistent with NS-culture-derived glioma cells with high invasiveness (Figure 3), genes highly expressed in 51A were those encoding markers of neural stem/precursor cells (NANOG, POU3F2, POU5F1, and SALL2), and pro-invasive proteins (AGAP2, EPHA2, FOXM1, PDGFRA, and TNC). This evidence concerns the gene POU5F1 and central nervous system cancer.