Low-grade astrocytomas and oligodendrogliomas demonstrate intermediate transcript expression of CASP3. To extend upon CASP3 gene expression data, protein levels of procaspase-3 in 10 glioblastoma cell lines (both serum-cultured and oncosphere lines) were analyzed by immunoblotting with an antibody recognizing procaspase-3. The gene discussed is CASP3; the disease is glioblastoma.