CD4 and infection: However, sorted rectal CD4+ T cells were negative for HIV RNA and DNA (collected 1.9 months after infection), and leukapheresis-collected PBMCs enriched for total CD4+ T cells and sorted CD4+ T cell subsets (TN, TCM, TTM, and TEM) were negative for cellular HIV RNA, total HIV DNA (confirmed in 2 independent laboratories), integrated HIV DNA, and 2-LTR circles (collected 2.1 months after infection).