THRAP3 and cancer: To examine the effect of these mutations (R101* and R837*) on cellular DNA repair and their potential role in cancer development, U2OS cells, which express endogenous wild-type (wt)-THRAP3, were transfected with wt or mutant THRAP3 constructs and cellular DNA repair capacity assessed, thus mimicking the heterozygous nature of these mutations in cancer cells (Figure 6A).