Quantitation of these blots showed a trend towards increased binding of both Aly and Ddx39b to the S85C and P154S mutations in Matrin 3, while increased binding of S85C Matrin 3 to Ddx39b reached statistical significance (Fig. 4e,f).Co-immunopreciptiation between Matrin 3 and Ddx39b was also performed in human post-mortem lumbar spinal cord tissue, confirming that these proteins interact in vivo and in the context of sporadic ALS (Fig. 4g). This evidence concerns the gene MATR3 and amyotrophic lateral sclerosis.