Here, we evaluated the efficacy, safety and proper dose of PEG-G-CSF in postoperative adjuvant ddEC-P chemotherapy ± trastuzumab among node positive Chinese breast cancer patients, and further explored the proper dosage of PEG-G-CSF during both ddEC treatment and ddP treatment ± trastuzumab regimen. This evidence concerns the gene CSF3 and breast carcinoma.