While CD45-negative, vimentin-positive sarcoma CTC nucleated cells were readily detected in mice with localized disease (all five mice euthanized prior to amputation) and in mice with metastatic relapse (all eleven mice which were euthanized on POD 30), there were substantially fewer CTC detected in the three mice which were evaluated on POD 9 (Figure 2E). Here, VIM is linked to sarcoma.