RNASE1 and cancer: Of the cancer-related signaling pathways, we detected a negative regulation of some tumor-promoting pathways in tumor tissue after RNase A treatment, including the PI3K/AKT (three upregulated and twelve downregulated genes), TGF-β (two upregulated and six downregulated genes), JAK/STAT (two upregulated and eleven downregulated genes), and canonical WNT signaling (six upregulated and eleven downregulated genes) pathways (Supplementary Tables 1 and 2, Supplementary Figures 2, 4, and 5).