In summary, our results demonstrated that, similar to the present immunosuppressant drug leflunomide, astilbin, isolated from GZ-SRG, significantly mitigated RA disease development in CFA-induced arthritic rats by effectively suppressing functionally activated cytokines and the key molecules that are associated with TLRs-NF-κB signaling of the inflammatory response pathway in RA disease process. The gene discussed is NFKB1; the disease is rheumatoid arthritis.