To complete these results at the protein level, we analyzed LC3B levels by western blot in ALS tibialis and soleus. Contrasting to the mRNA expression data, we found an accumulation of the two forms of LC3B, LC3B-I and LC3B-II, the lipidated version of LC3B-I involved in autophagosome formation, in ALS tibialis when compared to controls (Figure 5C and Supplementary Figure S3A). The gene discussed is MAP1LC3B; the disease is amyotrophic lateral sclerosis.