Increased expression of sulfatase in the neurospheres of primary cultures of human and mouse gliomas was associated with a low content of trisulfated disaccharides of HS in the system in vitro [15], while increased expression of SULF1 and SULF2 in brain cancer was associated with progression and prognosis of the disease in vivo [24]. This evidence concerns the gene SULF1 and glioma.