To further demonstrate the role and involvement of EPHA4 downstream of TGFβ- mediated cell migration in basal breast cancer, we infected MDA-MB-231 and SCP2 cells with lentiviruses overexpressing scrambled or EPHA4 shRNAs, in order to generate stable cells in which EPHA4 gene expression was specifically knockdown. Here, EPHA4 is linked to breast carcinoma.