In one of these HCC, 3 different subclonal mutations of CTNNB1 (cancer cell fraction = 5, 30, and 39%) affected the D32 and S33 hotspots (Fig. 4b) suggesting that the WNT/ß-catenin pathway activation was selected independently in several clones late during tumor progression in this patient. This evidence concerns the gene CTNNB1 and neoplasm.