With correlation of expression profiling of metabolic RLEs and differentiation status of TCGA-LIHC and HCC cell lines, we revealed that upregulated RLEs in pyrimidine metabolism especially the 3 RLEs (TK1, TYMS and DTYMK) of dTTP biosynthesis pathway play critical roles in increasing cellular dTTP concentration and sustaining the stemness properties. This evidence concerns the gene TYMS and hepatocellular carcinoma.