Four high-impact variants were identified within aggressive human tumor PCB439, one within NPIPB3, one within IPO7 and two within ABHD2. Notably, all human tumors contained high- or moderate- impact mutations within the largely uncharacterized gene NPIPB3. None of the genes with high-impact mutations found in curable PCB429 (ARHGAP32, HTRA1, LOC388813, OSGEPL1, PTEN, TAF8, TNKS) were mutated in either aggressive human sample. Here, TNKS is linked to neoplasm.