SIRT6 and hepatocellular carcinoma: The expression of SIRT6 was only detected in HepG2-SIRT6 and HCCLM3-SIRT6 cells by using primary anti-Flag antibody, but not in HepG2-3.1 and HCCLM3-3.1 cells (Figure 1A and 1B), which suggests that stable SIRT6-overexpressed HCC cell lines were successfully established.