To test the effects of SIRT6 overexpression on the capability of tumor growth in vivo, BALB/c nude mice xenograft models were established by subcutaneous injection with HepG2-SIRT6, HepG2-3.1, HCCLM3-SIRT6 and HCCLM3-3.1 cells. This evidence concerns the gene SIRT6 and neoplasm.