SIRT6 and neoplasm: The tumor weight was significantly decreased in both HepG2-SIRT6 (0.2555 ± 0.1295 g) and HCCLM3-SIRT6 (0.1814 ± 0.0934 g) groups compared with HepG2-3.1 (0.5849 ± 0.2699 g) and HCCLM3-3.1 (0.4898 ± 0.1455 g) groups, respectively (Figure 3A and 3B), which suggests that overexpression of SIRT6 significantly reduced tumor formation and tumor growth of HepG2 and HCCLM3 cells in vivo.