The results of the luciferase reporter assay suggest that transfection of miR-494-3p mimic into 293T human embryonic kidney cells or BT-474 breast cancer cells significantly reduced the activity of luciferase fused with wildtype 3′-UTR of BMI1, but not to the luciferase fused with mutated 3′-UTR by deletion of miR-494-3p binding sites (Figure 4A). This evidence concerns the gene BMI1 and breast cancer.