XIST specific short harpain RNAs (sh#1 and sh#2) and nonspecific short hairpin RNA used as negative control (NC) were transfected into KYSE30 and KYSE150 cells and subsequent qRT-PCR assays confirmed successful knockdown of XIST in ESCC cells (Figure 2A). This evidence concerns the gene XIST and esophageal squamous cell carcinoma.