With the onset of the national HIV program in South Africa and the development of strategic HIV and AIDS Plans for South Africa [8–10], the challenges of providing CD4 enumeration as a routine test across multiple laboratories, led to the local development of a low-cost, easy-to-use, single platform, 2-color, lyse-no-wash cytometry assay, i.e. the PanLeucogate (PLG) CD4 [11–13]. The gene discussed is CD4; the disease is AIDS.