To determine whether EFHD2 contributes to the metastatic abilities of lung adenocarcinoma cells, we used the pcDNA vector to overexpress EFHD2 in A549 cells (low endogenous EFHD2 levels; Fig. 1C) and shRNA to knockdown EFHD2 in H1299 cells (high endogenous EFHD2 levels; Fig. 1C). The gene discussed is EFHD2; the disease is lung adenocarcinoma.