Reminiscent of the sinus bradycardia of human R302Q PRKAG2 mutation carriers (Fig. 1a), invasive electrophysiology studies performed under isoflurane general anesthesia revealed a reduction in sinus HR of homozygous R299Q γ2 mice in vivo (Fig. 2a). This evidence concerns the gene PRKAG2 and Sinus bradycardia.