As AMPK has been reported to regulate autophagy, glucose uptake, and glycolysis (Hardie and Pan, 2002; Hardie et al., 2012), we next examined whether downregulation of AMPK‐α1 can abort glycolysis and ADI‐PEG20‐induced autophagy in parental melanoma cells as seen in BR cells. The gene discussed is PRKAA1; the disease is melanoma.