As AMPK has been reported to regulate autophagy, glucose uptake, and glycolysis (Hardie and Pan, 2002; Hardie et al., 2012), we next examined whether downregulation of AMPK‐α1 can abort glycolysis and ADI‐PEG20‐induced autophagy in parental melanoma cells as seen in BR cells. This evidence concerns the gene PRKAB1 and melanoma.