Furthermore, in an analysis by Rheinbay et al.30, deep sequencing of 360 primary breast cancers revealed six promoters (TBC1D12, LEPROTL1, ZNF143, RMRP, ALDOA and FOXA1) that contained single-site mutational hotspots (three or more mutations at a single site). This evidence concerns the gene TBC1D12 and breast carcinoma.