Although the population of immune cells in the peritoneum decreased by Cy treatment, E. cuniculi infection increased CD4+ and CD8+ T cells and also macrophages in Cy-Infected and DM-Cy-Infected mice compared to uninfected controls (Cy-Uninfected and DM-Cy-Uninfected), showing the importance of these cells against microsporidiosis, even in an immunosuppressive condition (Fig 7). This evidence concerns the gene CD4 and microsporidiosis.