Strong evidence of TNT formation in vitro (e.g., infectious disease, oncology, neurology, development)Intercellular communication /signaling/cargo/dyesInducible (infection/inflammation)A Large variety of cells capable of TNT formationSome evidence of TNT formation in vivo (oncology)Evidence that M-sec, myosins, F-actin, and calcium transfer are involvedShaking/physical disruption blocks TNT formationGap junctions may play a role in TNT connecting to receiving cells (Focus if this review). This evidence concerns the gene MYH14 and infection.