We found that 48 h post transfection, pAkt/AKT levels were significantly decreased in three CHL1 siRNA-transfected human glioma cell lines compared with cells transfected with negative control siRNA (p < 0.05 for both comparisons in both SHG44 and U-87 MG cells and p < 0.01 for both comparisons in U251 cells (Figure 8). This evidence concerns the gene CHL1 and central nervous system cancer.