Nuclear TOB1 expression was reduced in GC patients with lymph node metastasis (N0 vs. ≥ N1, P = 0.008), distant metastasis (M0 vs. M1, P = 0.007), and high TNM stage (I and II vs. III and IV, P = 0.043). Here, TOB1 is linked to metastatic malignant neoplasm in the lymph nodes.