To evaluate if observed effects on ATM activity in the nuclear extracts were recapitulated upon treatment of intact cells, HT29 colon cancer cells and MDA-MB-436 breast cancer cells were treated with CC-115 for 2 or 12 hours in the presence or absence of the DSB inducer bleomycin and the total cell lysate was used for immunoblotting (Figure 3D). This evidence concerns the gene ATM and colonic neoplasm.