In conclusion, the results of the present study suggest that complementing the immunohistochemical assesment of ERG with SLC45A3 and PTEN expression status (“triple hit” phenotype) identifies PrCa subgroups with different molecular pathways, and, because of the relationship of this combination of markers with tumor grade and biochemical progression, they can be used to improve patient stratification, treatment and follow-up. Here, ERG is linked to neoplasm.