ANXA5 and glioblastoma: SR144528 effectively decreased CBD-induced apoptosis at relatively early phase that was detected with Annexin-V-FITC and PI staining in both GBM lines 16 h after treatment (data not shown) and substantially downregulated total apoptotic (the sub-G1) levels 72 h after treatment determined by cell cycle and apoptosis analysis using PI-staining DNA and the flow cytometry (Figure 11D).