To further confirm that the inhibition of autophagy by CQ promoted NDV-activated apoptosis in CEF cells, we analyzed CEF cells that were pretreated with CQ (50 μM) prior to viral infection with NDV (MOI = 1) by annexin V–FITC/propidium iodide double-staining flow cytometry assay at 24 and 48 h. The gene discussed is ANXA5; the disease is viral infectious disease.