As seen in Figure 3A, s4428z-equipped T-cells secreted significant amounts of IFNγ, IL2, and TNFα when cocultured with cells from the primary melanoma culture M#3 (e.g., 3,987, 343, and 1,027 pg/ml, respectively), underscoring the potential clinical relevance of our approach. This evidence concerns the gene TNF and melanoma.