The goal of this study, therefore, is to evaluate the role of Nox4 in the LPS induced responses in a well-characterized human hepatoma cell line, primary hepatocytes isolated from control wildtype (Nox4+/+) and Nox4-deficient (Nox4−/−) mice, and in these animals in vivo. This evidence concerns the gene NOX4 and hepatocellular carcinoma.