In order to provide further insights into the nature of APRT as essential gene, we extended the analysis and conducted a more detailed in vitro silencing in several acute leukemia cell lines: CEMO-1, KG-1 (Acute myeloid leukemia (AML)), PEER, MOLT-4 (T-ALL), and MY (B-ALL). Here, APRT is linked to acute lymphoblastic leukemia.