TFF3 and Barrett esophagus: This is in keeping with previous studies that have reported a significant correlation between BE segment length and hypermethylation of other markers, such as AKAP12 and CHD13, for example.22 35 Furthermore, the sensitivity of TFF3 is also affected by the length of the Barrett’s oesophagus in the same cohort such that the overall sensitivity (79.9%) increased to 87.2% for patients with >3 cm circumferential Barrett’s oesophagus.17 Similarly, the sensitivity of our methylation signature increased from 82.2% to 95% for segments >3 cm (online supplementary figure 3).