TFPI2 and colorectal cancer: Similarly, TWIST1 is also known to be hypermethylated in various tumour types including bladder and colorectal cancers.42 43 We found that although TWIST1 was significantly hypermethylated in Barrett’s samples from both pilot and validation cohorts, the AUC was lower compared with TFPI2 (AUC=81.4% for TWIST1).