SAMHD1 and infection: To investigate which step of the virus life cycle was inhibited by ETO, and consequently rescued by SAMHD1 depletion, we infected MDM with HIV‐1 BaL with and without co‐infection with SIV VLP and isolated DNA 18 h post‐infection to measure late RT products (Fig 2D), 2LTR circles (Fig 2E) and integration products (Fig 2F) under conditions of FCS culture where SAMHD1 is phosphorylated in the absence of ETO.