Further, the HCCC and BRE cells transfected with miR-200b showed decreased cell vitality and proliferation (Fig. 2e), despite the treatment of TGF-β, which suggested that miR-200b could not only inhibit the EMT of tumor cell, but also impair the cell proliferation in CCA. The gene discussed is TGFB1; the disease is cholangiocarcinoma.