Here, we found higher levels of IL-6, TNF-α, and MCP-1 accompanying elevated levels of AST and ALT in the WTD group than in the control, which indicated ongoing NASH and liver injury, while dietary flaxseed oil significantly reduced plasma levels of IL-6, TNF-α, and MCP-1 initially increased by WTD. Here, IL6 is linked to metabolic dysfunction-associated steatohepatitis.