Figure 2 shows that the phosphorylation independent antibody Tau 5 reveals immunopositivity mostly in cytoplasm, while anti p-Tau antibodies, p-Tau (Ser396), p-Tau (Thr231), and p-Tau (Ser214) stained both the cytoplasm and the nucleus in a cognitive impaired subject. The gene discussed is MAPT; the disease is Cognitive impairment.